Antigen

Antigen Class

TFs and others

Antigen

PGR

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

ChIP-seq PR T0 rep1

cell line

T47D

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted from cells treated or not for 6hs with 50pM or 10nM R5020 and submitted to massive sequencing using the Solexa Genome Analyzer. RNA was isolated from cells with TRIzol reagent (Ambion), ethanol precipitated, and dissolved in sterile water. RNA concentration was measured with a Qubit fluorometer and RNA subjected to Bioanalyzer for quality control. Libraries were prepared using 1 μg of polyA+ RNA by PCR amplification of cDNA with bar-coded primers using the Illumina TruSeq kit at the CRG Genomic Facility. Libraries were sequenced using Illumina HIseq- 2500 to obtain pair-ended (PE) 100-base-long reads. ChIP assays were performed as described (Strutt and Paro, 1999)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

64982965

Reads aligned (%)

98.8

Duplicates removed (%)

11.6

Number of peaks

1145 (qval < 1E-05)

hg19

Number of total reads

64982965

Reads aligned (%)

97.8

Duplicates removed (%)

11.9

Number of peaks

564 (qval < 1E-05)